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KMID : 1145720140020020041
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Korean Herbal Medicine Informatics
2014 Volume.2 No. 2 p.41 ~ p.47
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Evaluation of Genetic Diversity of Polygonatum spp. by the Analysis of Simple Sequence Repeat (SSR)
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Kim Wook-Jin
Ji Yun-Ui
Kang Young-Min
Moon Byeong-Cheol
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Abstract
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Purpose : To evaluate the genetic diversity and relationship of Polygonati Odorati Rhizoma and Polygonati Rhizoma in Polygonatum species, we carried out the single sequence repeat (SSR) analysis using 8 species 24 germplasm lines collected from different habitat in Korea and China.
Materials and Methods : Materials were collected randomly from different geographic regions in Korea and China, and were analyzed simple sequence repeat (SSR) of 120 primer sets which were designed based on next generation sequence (NGS) data. Total bands amplified by SSR primer sets were used to analyze genetic relationship using the unweight pair group method with arithmetic averages (UPGMA).
Results : SSR results of 17 primer sets were revealed 68 total band and 57 polymorphic band with a 83.8% ratio of polymorphism. 24 Polygonatum germplasm lines were ranged from 0.62 to 0.95 and clustered distinct three groups depending on the species with 0.66 coefficient value.
Conclusion : In this study, 17 SSR primer sets proper to evaluate genetic diverstiy of 8 Polygonatum spp. were selected. 5 species belonging the original plant of Polygonati Odorati Rhizoma, except P. inflatum Kom., were clustered into Group II. P. falcatum A.Gray were classified into Group I and P. sibiricum F.Delaroche were classified into Group III, respectively. These result showed that original plant of Polygonati Rhizoma were genetically different compared to Polygonati Odorati Rhizoma.
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KEYWORD
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Polygonatum spp., SSR, Genetic Diversity, Polygonati Rhizoma, Polygonati Odorati Rhizoma
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